Thp1 transwell
WebPromotional Article Monitoring. Register your specific details and specific drugs of interest and we will match the information you provide to articles from our extensive database and email PDF copies to you promptly. WebT1 - Differentiation of THP1 Cells into Macrophages for Transwell Co-culture Assay with Melanoma Cells. AU - Smith, Michael. AU - Young, Helen. AU - Hurlstone, Adam. AU - …
Thp1 transwell
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WebDay 1 THP-1 cells are grown in suspension in RPMI/FCS media, and activation is performed once plated into transwell inserts. Inserts are handled at the sterile edges of the inserts … WebMay 14, 2013 · This study was designed to identify metalloproteinase determinants of macrophage migration and led to the specific hypothesis that matrix metalloproteinase …
WebTranswell permeable supports are convenient and easy-to-use devices for studies of both anchorage-dependent and anchorage-independent cells. These inserts provide … WebApr 30, 2024 · We then used the THP-1 cell line, which could be primed from monocytes to become macrophage-like cells, for the Transwell assays. Conditioned media (CM) from …
WebMar 30, 2024 · Last, transwell migration assays showed that IL-1β-primed SKOV3-TCM significantly increased the recruitment of THP1/PBMC Mφ, compared to control TCM and … WebFigure Legend Snippet: The metastasis-related effects of conditioned medium derived from M2 macrophages. a A549 cells were treated with RPMI or conditioned medium derived …
Webthe migration of another THP1 monocyte cell line that stably expresses GFP for visualization.41 The top chambers of the transwells were filled with equal numbers of a …
WebMay 14, 2013 · This study was designed to identify metalloproteinase determinants of macrophage migration and led to the specific hypothesis that matrix metalloproteinase 10 (MMP10/stromelysin-2) facilitates macrophage migration. We first profiled expression of all MMPs in LPS-stimulated primary murine bone marrow-derived macrophages and … mallard duck decoys for saleWebApr 10, 2024 · We observed that THP-1 cell morphology shifted from round to irregularly shaped after PMA induction, indicating a change from suspension cells to adherent cells (Fig. S1A). The induction of THP-1 ... mallard duck eatingWebFigure 2. Human Monocytic THP-1 Chemotaxis. THP-1 cells were allowed to migrate toward MCP-1 for 2 hrs, 400,000 cells were used in each assay. Migratory cells were quantified by … mallard duck eggs for hatchingWebMay 7, 2024 · PMA induced the differentiation of THP-1 cells into M2-like TAMs. (a) THP-1 cells morphology after PMA treatment. Scale bar, 50 µm. (b) Expression of cell markers, … mallard duck decoy paint schemeWebFeb 8, 2006 · A higher number of RA FLS co-cultured with differentiated THP-1 cells (1055.67 ± 63.06 cells/filter) were found to have invaded through transwell chambers than that of RA FLS co-cultured with undifferentiated THP-1 cells (726 ± 83.86 cells/filter) (P < 0.05; Figure 4b,d). mallard duck classWebTranswell细胞迁移实验. 实验介绍. 细胞迁移与侵袭实验将 Transwell 小室放入培养板中,小室内称上室,培养板内称下室,上下层培养液以聚碳酸酯膜相隔,将研究的细胞种在上室内,由于 … mallard duck distributionWebDec 1, 2024 · To initiate the co-culture, the transwell filters with Caco-2 cells were transferred onto well plates containing 24 h-differentiated THP-1 cells without additional … mallard duck egg incubation length