WebAug 11, 2024 · Next, variants of T2A-iCre-ER T2 were constructed, with variations at the N-terminus of iCre-ER T2 after the ribosome skip. The sfGFP-iCre-ER T2 was included as a control. The mTagBFP2 was initially cloned in as a transfection marker to normalize for transfection efficiency. WebMar 8, 2024 · Published: 08 March 2024 Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background Lech...

Pearson Professional Center tour // Pearson VUE

WebMar 3, 2024 · zsGreen–T2A–CreERT2–SV40 poly(A)–LoxP–Neo–LoxP, was constructed by Gibson cloning (Gibson et al., 2009). This cassette was then used to replace the Atoh7 ORF in a genomic DNA fragment captured by BAC recombineering (Fu et al., 2009). The final construct contains a 5.3 kb 5’ arm, the zsGreen–T2A–CreERT2–SV40 poly(A)–LoxP– WebpCAGGS-mTagBFP2-T2A-iCreERT2 (PV) Plasmid #128171 Purpose Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus) Depositor Mario Capecchi Insert mTagBFP2 … ontario registry office online

Fate-mapping mice: new tools and technology for immune discovery

WebTake a photo tour. Take a walk through a Pearson Professional Center. The gallery of photos below introduces you to the physical layout of the testing center as well as the check-in … WebThe Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the … WebJul 12, 2024 · Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array … ontario regulation 153 04 tables