WebI. BioLogic DuoFlow System Section 1. DuoFlow System Preparation When the DuoFlow system is turned on, the Manual screen is displayed (see Figures 1 and 2). This screen displays instrument control panels that provide direct control of the pumps, valves, fraction collector, UV detector, QuadTec™ UV/Vis detector, and Econo™ Gradient Pump. WebFeb 15, 2024 · Experimental schematic. The experimental workflow of polysome profiling from mammalian tissues or isolated cell-types following by next-generation sequencing …
Cell-type specific polysome profiling from mammalian tissues
WebOct 7, 2016 · The polysome profile of fertilized embryos and the corresponding RNA profile of the 15–40% sucrose gradient separated on an agarose gel are shown in Figure 3B. In … WebThe mRNAs present in the polysome fraction are recruited more often by the translational apperature of the cell. A shift of a given mRNA into the polysomal fraction is often connected with higher ... platform 2022
Leading Life Science Research & Clinical Diagnostics — Bio-Rad
WebJan 1, 2024 · Polysome-profiling is commonly used to study translatomes and applies laborious extraction of efficiently translated mRNA ... or the Biologic LP pump (Bio-Rad) coupled to a Model EM-1 Econo UV detector (BioRad). Fractions (∼500 μl) were collected with either a Piston Gradient Fractionator (BioComp) coupled with a fraction ... WebOct 23, 2024 · Abstract. Polysome-profiling is commonly used to study translatomes and applies laborious extraction of efficiently translated mRNA (associated with >3 ribosomes) from a large volume across many fractions. This property makes polysome-profiling inconvenient for larger experimental designs or samples with low RNA amounts. WebThe BioLogic DuoFlow F10 workstation includes an F10 pump for a flow rate of 0.01–10 ml/min* at 3,500 psi (233 bar, 23 MPa). BioLogic DuoFlow F40 Workstation. The BioLogic … platform 2103