Phee401e-mcherry

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August undefined, 2024

WebJun 22, 2024 · Europe PMC is an archive of life sciences journal literature. WebThe pHEE401E_UBQ_Bar vector is a version in which the egg-speciﬁc promoter for Cas9 expression in the pHEE401E vector was replaced with the UBQ10 promoter, and the hygromycin resistance gene was ...

On Improving CRISPR for Editing Plant Genes: Ribozyme …

WebmCherry Antibody (M11217) in ICC/IF. Immunofluorescent analysis of mCherry Tag was performed using 70% confluent mCherry-H3 transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. WebHave a question, comment, or need assistance? Send us a message or call (630) 833-0300. Will call available at our Chicago location Mon-Fri 7:00am–6:00pm and Sat … hemnes sofa bed

A novel CRISPR/Cas9 system for efficiently generating Cas9-free ...

WebJan 6, 2024 · Both the first and second constructs were cloned in the pHEE401E vector, which contains a maize codon-optimized Cas9 gene (zCas9) driven by an Arabidopsis egg-cell specific promoter (E.C.1.1) fused with an egg-cell specific enhancer (E.C.1.2) [ 21 ]. Fig. 1 Schematic illustration of gRNA target sites and the two CRISPR multiplexing constructs. a. WebpHEE401E (~100 ng/μl) 2 μl 10× T4 DNA Ligase Buffer (NEB) 1.5 μl 10× BSA 1.5 μl BsaI (NE B) 1 μl T4 DNA Ligase (HC, NEB) 1 μl ddH 2 O 6 μl Total volume 15 μl 5. Transform E.coli … WebAn mCherry gene under the control of a seed-specific At2S3 promoter was cloned into the plasmid pHEE401E to generate pHEE401E-mCherry14,16. The two gRNA units, which … land wholesale contract

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Plasmid construction —Bio-101

WebIn addition, the overexpression construct will be transformed into the Arabidopsis ap1-1 cal-1 mutant plants by floral dipping method. We will also knock out the Arabidopsis … WebTo test whether the introduction of GFP-type termini into mRFP variants would benefit fusion proteins expressed in mammalian cells, we fused mRFP1 and mCherry to the N terminus of α-tubulin. In most HeLa cells, expression of mRFP1-α-tubulin resulted in diffuse cytoplasmic fluorescence rather than proper incorporation into microtubules.However, mCherry-α … land white mountainsWebDec 7, 2024 · 56 usually between 30% and 40% of successful protein expression in E. coli [18]. However, when mCherry 57 is used as a reporter, we observe fluorescence in about 65% of E. coli clones (Supplemental Figure S1). 58 Additionally, a large-scale analysis of the transcription start sites (TSS) of these clones showed a non- hemnes sofa table

"WebpHEE401E质粒价格1200元，pHEE401E(addgene#71287 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为卡那霉素，测序引物为M13-fwd。 pHEE401E质粒是植物 … " - Phee401e-mcherry

Phee401e-mcherry

On Improving CRISPR for Editing Plant Genes: Ribozyme …

WebJun 22, 2024 · The GFP gene is designed to fuse with part of CRY1 in frame, providing a visual marker for precise insertion of the gene drive element. The mCherry is another … WebNov 20, 2024 · The CRISPR genome-editing system includes two key components: an sgRNA that specifically recognizes the target DNA, and a Cas9 endonuclease that precisely …

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WebJun 22, 2024 · The GFP gene is designed to fuse with part of CRY1 in frame, providing a visual marker for precise insertion of the gene drive element. The mCherry is another … WebAug 16, 2024 · Building on the pHEE401E plasmid, a Cambia T-DNA adapted for 96 genome editing with CRISPR/Cas9 nucleases (Wang & al., 2015), we created a series of 97 vectors enabling selection and counter-selection of transgenics on the basis of seed 98 fluorescence. Toward this end, we replaced the hygromycin resistance marker of pHEE401E

http://www.biofeng.com/adjy/zhili/pHEE401E.html Web提取T 1 代植株基因组进行PCR鉴定和测序分析，鉴定纯合突变后代的性状表型及激素测定。结果表明，本实验成功构建了编辑载体pHEE401E- mCherry - CKX3，拟南芥转化后代中目标基因成功被高效编辑， CKX3 的纯合突变植株表型与野生型差异明显，内源激素含量差异达到显著水平。为研究 CKX 基因功能奠定了基础。关键词: 细胞分裂素, 氧化酶, 脱氢酶, 基因 …

WebJul 21, 2015 · Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 … WebAug 16, 2024 · Building on the pHEE401E plasmid, a Cambia T-DNA adapted for 96 genome editing with CRISPR/Cas9 nucleases (Wang & al., 2015), we created a series of 97 vectors …

WebAn mCherry gene under the control of a seed-specific At2S3 promoter was cloned into the plasmid pHEE401E to generate pHEE401E-mCherry14,16. The two gRNA units, which produce one gRNA from the Arabidopsis U6-26 promoter and one gRNA from the U6-29 promoter, were cloned into the BsaI site in pHEE401E-mCherry by Gibson assembly. We …

WebChen pHEE401E U6 -26p BsaI yes, cut S. pyogenes Hygro Chen pHDE-35S-Cas9-mCherry-UBQ U6 -26 PmeI yes, cut...opposite strand) mutations. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...homology-directed repair (HDR). landwick farmWebApr 18, 2015 · Notifications Code master crispr_cas9_arabidopsis/plasmids/pHEE401E.gbk Go to file Cannot retrieve contributors at this time executable file 389 lines (388 sloc) 24.9 KB Raw Blame LOCUS pHEE401E 17112 bp DNA circular 18-APR-2015 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ FEATURES … land white mountains nhWebJan 1, 2024 · The mCherry-based method provides an efficient and reliable approach to quickly identify transgene-free plants whose genomes have been properly edited. … land wichita