Web31 mrt. 2016 · HTGTS and LAM-HTGTS can both be used to detect DSBs generated from the cellular environment (e.g., ionizing radiation, chemotherapeutics, viral integration) 6,9,13. Web3 mei 2024 · LAM-HTGTS was primarily designed to identify rare DNA double-strand breaks genome-wide 19. Although it has considerable merit for this application, its adaptation for repertoire sequencing, in...

Unbiased quantification of immunoglobulin diversity at the …

Web15 dec. 2014 · The HTGTS 23 and translocation-capture sequencing approaches 24 were developed to identify translocations of yeast I-SceI meganuclease-generated 'bait' DSBs … WebWe modified the LAM-HTGTS protocol by utilizing CRISPR-Cas9 to create the double-strand DNA break at a specific site and also added a step with a 5’ methyl cytosine modified primer to promote LpnPI endonuclease cleavage … photographing art for prints

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Web15 mrt. 2024 · Extraction protocol: Rapid lysis buffer + 10ug/ml Proteinase K, at 56°C, overnight See Supplemental Experimental procedures from Chiarle et al. Cell 2011, paragraph Generation of HTGTS libraries by adapter-PCR Gennomic DNAs were extracte with Rapid lysis buffer containing 20 ug/ml of proteinase K overnight at 56°C. WebHTGTS was developed to study translocation mechanisms in mammalian cells. This approach is particularly suitable for studying for AID-dependent IgH class-switching … Web26 aug. 2015 · To confirm DC–PCR findings and analyse potential mechanisms, we used high-throughput genome-wide translocation sequencing (HTGTS), an unbiased genome-wide approach that identifies ‘prey’ DSB... how download shaders in minecraft