Fast halo assay
WebJan 1, 2009 · The fast-halo assay (FHA) is a very recent method to detect DNA-strand breakage induced either by various genotoxic agents or secondary to apoptotic DNA … WebSep 5, 2006 · The results obtained allow us to present a method named fast halo assay (FHA), which can operate both at denaturing and – with slight modifications – non …
Fast halo assay
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WebMay 24, 2024 · The halo assay also enables high throughput measurement (96 samples instead of 4) using 25 µL of whole blood per sample, compared to 300 µL for viscoelastic based methods, which would offer ... WebSep 5, 2006 · The results obtained allow us to present a method named fast halo assay (FHA), which can operate both at denaturing and – with slight modifications – non-denaturing conditions and is characterized, as compared with the original AHA, by a two-third reduction of total processing time, a simplification of reagent requirements, and a …
WebJul 15, 2024 · This permits the rapid assessment of the extent of DNA breakage caused by different types of DNA lesions. FHA has proven to be sensitive, reliable, and flexible. … WebNov 5, 2024 · DNA damage was also evaluated using the Fast-Halo assay. In vivo efficacy was assessed in NSG mice systematically engrafted with stably luciferase transfected Molm13 BLIV cells via tail vein injection. Cohorts of mice (5-10) were treated with vehicle, Ven (100 mg/kg given 5 days/week for 3 weeks), Tala (0.33 mg/kg for 5 days/week over …
WebFast halo assay was used to investigate nuclear damage; quantitative polymerase chain reaction (QPCR), standard PCR, and real-time PCR were used to evaluate mitochondrial DNA integrity, content, and gene expression. HUVECs were continually exposed to a 300 mT SMF for 4, 24, 48, and 72 h. Compared to control samples (unexposed cultures) the … WebJul 1, 2011 · The fast-halo assay (FHA) is as sensitive and reliable method for detection of DNA strand breakage as the comet assay. But it has the advantage over comet assay …
WebSep 7, 2014 · Halo assay is another technique to measure DNA damage. The term “halo” refers to the shape of radially extracted DNA fragments from isolated nuclei. The …
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