Enliten atp assay promega madison wi
Webendogenous ATP and the accessibility of exogenous ATP to the luminal side of the endoplasmic reticulum (ER) where the active site of UGT is located. Gefitinib, This article … WebAug 30, 2024 · We used an ATP assay kit (Enliten, Promega, Madison, WI, USA) to estimate the ATP concentration in the supernatant by measuring in the luminescence channel of a Cytation 5 plate reader (BioTek Instruments, Inc., Winooski, VT, USA). The results are expressed in nanomolar ATP per milligram of proteins.
Enliten atp assay promega madison wi
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WebDec 15, 2024 · Supernatant was collected and analyzed using an ENLITEN ATP assay (Promega, Madison, WI, USA) and HMGB1 ELISA kit II (Shino-Test, Kanagawa, Japan). Western blotting. Cells were seeded in a 100-mm dish at a density of 1 × 10 6 cells/dish 24 h before viral infection. Murine and human PDAC cells were infected with OBP-301, Ad … WebNext, 10 mL of each sample were dispensed in a blank 96-well plate and 90 mL of luciferin/luciferase reagent were added (Enliten ATP Assay System; Promega, Madison, WI, USA). Bioluminescent signals were immediately detected with a luminometer (Glomax Luminometer; Promega, Madison, WI, USA).
WebFF2000 ENLITEN® ATP Assay System Components: F201 ATP-Free Water ... Madison, WI 53711 U.S.A. 1-608-274-4330 1-800-356-9526 SDS author: [email protected] 可获取更多资料的部门: Promega (Beijing) Biotech Co., Ltd Global Trade Center, B907 - 909 36 Eastern Road, 3rd North Third Ring WebGloMax® 20/20 Luminometer and the ENLITEN ATP Assay System. 10 μL of ATP Standard diluted in HEPES buffer was added to a test tube containing 100 μL rL/L Reagent. When designing your ATP assay with the ENLITEN Kit, it is important to remember several key aspects of the luciferase reaction. First, the rL/L reacts optimally at a pH of 7.73
Web2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB306 · Revised 5/09 www.promega.com 1. Description The CytoTox-ONE™ Homogeneous Membrane Integrity Assay(a) is a homogeneous, fluorometric method for estimating the number of … WebPromega ENLITEN® ATP Assay INTRODUCTION The Turner BioSystems Veritas™ Microplate Luminometer combined with the Promega ENLITEN® ATP Assay System …
WebMay 9, 2024 · After being treated for different time points (0.5, 1, 1.5, and 2 h), the supernatant of cells was analyzed using the ENLITEN ATP luciferase assay kit …
WebVarious pain therapies have been developed on the basis of the gate control theory of pain, which postulates that nonpainful sensory inputs mediated by large-diameter afferent … the devil wears prada albums rankedWebATP bioluminescence. ATP releasing agent (Somalyze), Tris-acetate buffer, ATP-monitoring reagent (firefly luciferase, D-luciferin) and ATP-standard (EG+G. Wallac, Turku, Finland) are needed for the assay. 100 µL of ATP releasing agent was added to each well of a 96 well microtitre tissue culture plate (Greiner, Germany), containing 100 µL of ... the devil wears prada analysisWebJan 1, 2016 · The total volume was adjusted to 1 ml in each condition. The amount of HMGB1 and ATP in the culture medium was measured using an HMGB1 enzyme-linked immunosorbent assay II kit (SHINO-TEST, Tokyo, Japan) and a luciferin-based ENLITEN ATP assay (Promega, Madison, WI), respectively, according to each manufacturer’s … the devil wears prada album coverWebNext, 10 mL of each sample were dispensed in a blank 96-well plate and 90 mL of luciferin/luciferase reagent were added (Enliten ATP Assay System; Promega, … the devil wears prada andy and christianWebendogenous ATP and the accessibility of exogenous ATP to the luminal side of the endoplasmic reticulum (ER) where the active site of UGT is located. Gefitinib, This article has not been copyedited and formatted. The final version may differ from this version. DMD Fast Forward. Published on July 30, 2012 as DOI: 10.1124/dmd.112.046862 the devil wears prada andy before and afterWeband the ENLITEN® ATP Assay System. Ten microliters of ATP Standard diluted in ATP-free water was added to a microcentrifuge tube containing 100 μL of rL/L Reagent. When designing your ATP assay with the ENLITEN kit, it is important to remember several key aspects of the luciferase reaction. First, the rL/L reacts optimally at a pH of 7.73 the devil wears prada authorWebENLITEN® ATP Assay System 100 assays ₩ 377,000 Your price: Log in The ENLITEN® ATP Assay System can be used to measure ATP levels for the indirect detection of biocontamination on food processing surfaces, in cosmetics and beverages or to assay for enzymes that degrade ATP and to quantitate ATP in biological fluids. the devil wears prada apa citation