Cyofp
WebCas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Lower activity than other Cas9 strains, but ... WebNov 8, 2024 · We injected targeting plasmids specifying GFP insertion at five other synaptic genes previously used to assay insertion frequency using array-borne Cas9 [ 12 ]. For each locus, the targeting reagents comprise …
Cyofp
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WebMay 29, 2015 · A Guide to Fluorescent Protein FRET Pairs. Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors … WebAbstract. The invention provides large stokes displacement fluorescent protein CyOFP and application of the fluorescent protein to microimaging and highly-sensitive living creature …
WebMost of these have a much brighter pharyngeal cyOFP marker to track the landing site (which is lost during insertion). These landing sites do not provide additional chromosomal locations for insertions, but they should be easier to use if you do not have a fluorescent dissecting scope with a high power objective. Web本发明提供了一种大斯托克斯位移荧光蛋白CyOFP以及该荧光蛋白在显微成像和高灵敏活体生物发光成像中的应用 ...
WebStanford researchers have engineered an exceptionally bright, cyan-excitable orange-red fluorescent protein (CyOFP) that can be used both for multiplex imaging with GFP and for high-sensitivity, bioluminescent in vivo imaging. Web一株指示牛免疫缺陷病毒BIV感染的细胞系及其应用。该细胞系可以在BIV感染的特定条件下表达增强型绿色荧光蛋白（EGFP），从而通过检测该蛋白的表达来间接指示牛免疫缺陷病毒的感染。该细胞系（其保藏号为CGMCC No．2050）的构建过程：首先构建成一个可以应答牛免疫缺陷病毒感染的具有序列表所 ...
WebSep 28, 2001 · The bph genes in Pseudomonas sp. KKS102, which are involved in the degradation of polychlorinated biphenyl/biphenyl, are induced in the presence of biphenyl. In this study our goal was to understand the regulatory mechanisms involved in the inducible expression. The bph genes (bphEGF(orf4)A1A2A3BCD( …
WebInserted into F53A2.9. High Cas9 activity. Superficially wild-type. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift … ironman memorial cityWebPlasmid pAS1-rab-3P::his-24::CyOFP from Dr. Andrew Leifer's lab contains the insert rab-3, CyOFP. This plasmid is available through Addgene. port washington police departmentWebAbstract. Sensitive detection of two biological events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal substrate specificity to firefly luciferase (FLuc) and its derivatives such as AkaLuc. ironman meets spiderman the avengers comicWebJul 1, 2024 · The construct Antares, made by fusing NanoLuc to the cyan-excitable orange-red fluorescent protein CyOFP, combines the high catalytic rate of NanoLuc with the high quantum yield and red-shifted... ironman minigame teleportWebOur work includes the creation of a series of fluorescent proteins with improved brightness or red-shifted spectra, including mClover1-mClover3, mRuby2-mRuby3, CyOFP, mCyRFP1, mCyRFP3, mCardinal, mMaroon1, and mMaroon2. By creating a new reporter of S-phase exit and by performing four-color imaging with mMaroon1, we developed the FUCCI4 … port washington pre-kWebAfter the CyOFP-conjugated R3/I5 bound to a shortened human RXFP3 (removal of 33 N-terminal residues) fused with the NanoLuc reporter at the N-terminus, high BRET signals … ironman memory foam 1000 inversion tableWebCyOFP1-TEV Expression Mammalian Mutation Promoter Availability Academic Institutions and Nonprofits only Enlarge pKK-TEV-CyOFP1 Plasmid #105799 Purpose Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging. Depositor ironman memory foam system 1000