Cyfbpase
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Cyfbpase
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WebDec 13, 2024 · CyFBPase plays a regulatory role in gluconeogenesis and is strongly inhibited by adenosine monophosphate (AMP) and fructose-1,6-diphosphate; on the contrary, cpFBPase plays a regulatory role in photosynthesis and is not sensitive to AMP and fructose-1,6-diphosphate [ 14 ]. WebMar 1, 2003 · The recombinant cyFBPase was expressed in E. coli BL21 (DE3) cells in a soluble form and purified to homogeneity by Ni +–NTA affinity chromatography. The identity of the recombinant cyFBPase was confirmed by SDS–PAGE and immunoblot analysis using a polyclonal anti-His tag antibody.
WebOne of the enzymes involved in the synthesis of sucrose in the cytoplasm of palisade mesophyll cells is known as cyFBPase. The gene coding for this enzyme is cyFBP. The … WebThe recombinant cyFBPase was active at neutral pH ranges (6.6-9.0) and thermostable as other cyFBPases. The activation energy (Ea) and Arrhenius frequency factor were 17.4 kcal/mol and 2.6 × 1012/s, respectively. The KM and Vmax values of the recombinant enzyme were calculated as 10.47 μM and 109 μmol/min, respectively.
Webbisphosphatase (cyFBPase), sucrose phosphate synthase (SPS, EC 2.1.4.14), sucrose synthase (SuSy, EC 2.4.1.13), and cell wall invertase (CIN) (Winter 2000). Among these enzymes, SPS and cyFBPase are responsible for irre-versible sucrose synthesis from UDP-glucose and fructose-6-phosphate (Serrato et al. 2009). SuSy catalyzes the WebThe cyFBPase gene expression and enzyme activity were increased significantly in transgenic plants, with 1.88 times and 1.55 times of the wild type controls respectively, and at the same time, the sucrose content in transgenic plants leaves was also significantly increased to 1.59 times than that of the wild type. Under the condition of drought ...
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WebJun 1, 2009 · In addition to the cytosolic enzyme (cyFBPase) present both in prokaryotic and eukaryotic cells, which participates in gluconeogenesis and sucrose synthesis … dgca directory pdfWebplant cells. In addition to the cytosolic enzyme (cyFBPase) present both in prokaryotic and eukaryotic cells,which par-ticipates in gluconeogenesis and sucrose synthesis (Cséke & … cibao international airport car rentalWebElevated activities of cytosolic fructose-1,6-bisphosphatase (cyFBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape (Brassica napus) cDNA in tobacco (Nicotiana tabacum) plants. ciba manchesterWebCytosolic fructose-1,6-bisphosphatase (FBPase) appears to be a key enzyme in gluconeogenesis and position branch point of carbon partitioning between paramylon and wax ester biosynthesis. We herein identified and … cibao intl airportWebMar 5, 2015 · (C) Western blot analysis of crude leaf extracts of cyfbp, cpfbp1, and cyfbp cfbp1 mutants and the wt. Proteins (25 μg) were separated by SDS–PAGEs, transferred to nitrocellulose filters, and immunolabelled with rabbit antiserum raised against cyFBPase and cFBPase1 antibodies (see the Materials and methods). Bands are ~40kDa, and … dgca approved drones in indiaWebTwo FBPase isozymes have been identified in eukaryotic cells of photosynthesis, with one in the cytoplasm (cyFBPase), the key enzyme of gluconeogenesis, and the other one in the chloroplast... cibap creatief vakmanWebDownload scientific diagram Changes of carbon partitioning in Arabidopsis plants with low (A) cyFBPase or (B) SPS activity (Strand et al. , 2000). from publication: SerratoJXB09 ResearchGate ... dgca class 2 medical renewal