Ctsk macrophage

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August undefined, 2024

WebApr 13, 2024 · On day 5, the expression of nuclear factor of activated T cell 1 (NFATc1), acid phosphatase 5 (Acp5), cathepsin K (Ctsk) and matrix metalloprotein 9 (Mmp9), marker genes of osteoclast activity ... WebLung macrophages utilize unique cathepsin K-dependent phagosomal machinery to degrade intracellular collagen. Cathepsin K regulates the tumor growth and metastasis …

Obesity, but not high-fat diet, is associated with bone loss that is ...

WebJun 5, 2024 · CTSK and MMP9 transcripts have also been found to be part of a macrophage multinucleation network in the rat (Kang et al., 2014). In addition, MGCs have been shown to express TRAP ( Park et al., 2012 ), suggesting that phenotypic markers of cell multinucleation are shared by MGCs and osteoclasts. WebJul 17, 2013 · Shp2 levels were reduced by >80% in bone-marrow-derived macrophages and osteoclasts in LysM-KO and Ctsk-KO mice (Supplementary Fig. 1a and data not shown). LysM-KO and Ctsk-KO … easetailored

Cathepsin K Knockout Exacerbates Haemorrhagic Transformation …

WebMay 18, 2012 · Other CtsK-Cre lines have also been reported that are not knock-in lines, but based on Ctsk-Cre transgenic overexpression [50, 51]. These have been used for deletion of Fcgr4 [52], Vdr [53], and ... WebMay 7, 2024 · After knockout of Ctsk, the gene encoding Ctsk, the outcomes of Ctsk +/+ and Ctsk −/− mice were compared 24 h after rtPA-treated cerebral ischaemia with respect to HT severity, neurological deficits, brain oedema, infarct volume, number of apoptotic neurons and activated microglia/macrophage, blood–brain barrier integrity, vascular ... WebThe activation of NFATc1, the master of osteoclastogenic transcription factor, directly regulates the induction of many osteoclastic genes, including cathepsin K (CtsK), matrix me- talloproteinase-9 (MMP-9), osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP), and dendritic cell–specific transmembrane protein ... ease systems

Cathepsin K--a marker of macrophage differentiation? - PubMed

Free Cholesterol Accumulation in Macrophage Membranes …

WebJan 16, 2013 · Mx1-Cre–induced deletion of Ctsk in hematopoietic cells and osteoclasts increased osteoclast differentiation from BM macrophages (BMMs) (Figure 6, ... WebJul 13, 2024 · Cathepsin K (Ctsk) is a cysteine protease of the papain superfamily initially identified in differentiated osteoclasts; it plays a critical role in degrading the bone matrix. However, subsequent in vivo and in vitro studies based on animal models elucidate novel subpopulations of Ctsk-expressing cells, which display markers and properties of … ease s winsor newtonWebMay 2, 2024 · Notably, macrophages contribute to the extracellular release in our models as osteoclasts are a macrophage derivative and cathepsin K is a macrophage maturation marker 43. Inside the cells ... ct townhouse investment

"WebSep 22, 2024 · All the genes were classified in order to clarify whether CTSK and M2 macrophages were correlated in CRPC. Before establishing the co-expression matrix withed weight, we defined the soft-threshold β as 6 in order to guarantee a scale-free topology. The degree of independence was 0.8 when the soft-threshold β reached 6 (Fig. … " - Ctsk macrophage

Ctsk macrophage

JCI - Osteoclast-specific cathepsin K deletion stimulates …

WebApr 5, 2024 · Importantly, receptor activator of NF-κB (RANK) ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) regulate osteoclastic differentiation and function ... Treatment with RANKL significantly increased the expression of DC-Stamp, TRAP, and Ctsk at 4 days, which was suppressed by RhoA silencing (Additional file 1: … WebSep 2, 2024 · However, at 34 hphs, the motility of differentiating macrophages became reduced as they started to express ctsk:GFP, while undifferentiated macrophages remained highly mobile (Supplementary Fig. S1B; Movie S4). This suggests that the reduction of macrophage dynamics is a prerequisite for osteoclast differentiation, …

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WebMay 21, 2012 · CTSK KO macrophages are less invasive than WT cells. The ability to invade surrounding matrix is another key factor affecting macrophage infiltration into the … WebRepresentative images of Ctsk-Cre/mTmG macrophages and osteoclasts captured on the indicated days (Day 0 is the time RANKL was first added to osteoclast cultures). Red and green channels supposedly correspond to the indicated fluorophores. The scale bar corresponds to 100 μm and applies to all images.

WebSep 22, 2024 · Fig. 7: High expression of CTSK is related to high enrichment of M2 macrophages of tumor-associated in CRPC. A – D IF assay analysis of the correlation … Web上海纪宁实业有限公司成立于上海，在上海张江高科园区拥有独立实验室，公司主力提供的生化产品有：酶联免疫试剂盒（elisa试剂盒）、标准品、对照品、抗体、生化试剂、培养基、血清、细胞株、金标试剂盒等试剂产品。

WebMar 29, 2024 · CTSK cathepsin K Gene ID: 1513, updated on 29-Mar-2024 Gene type: protein coding Also known as: ... Cathepsin K regulates the tumor growth and metastasis by IL-17/CTSK/EMT axis and mediates M2 macrophage polarization in castration-resistant prostate cancer. Wu N, Wang Y, Wang K, Zhong B, Liao Y, Liang J, Jiang NWu N, et al. … WebWe previously found increased Ctsk expression and secretion in Npc1 / macrophages and increased elastase activity in aortic homogenates of Apoe /, Npc1 / mice.5 To confirm in vivo relevance, we used an affinity-purified antibody18 and found strong CATK immunostaining in the macrophage-rich regions of atherosclerotic plaques in Apoe /, Npc1 mice

WebApr 4, 2024 · Conditional ablation of PDGF-BB in macrophage-lineage TRAP + cells significantly reduced expression of periostin in periosteum, and an increase of PDGF-BB in Ctsk –/– mice significantly upregulated periostin expression (Figure 6, A and B), suggesting that PDGF-BB induces the periosteal osteogenic microenvironment on the periosteal …

WebAug 20, 2024 · Then, the pretreated macrophages were adoptively transferred into macrophage-depleted mice as shown in Fig. 2h. Results indicated that the mice transferred with S5-pretreated M1-BMDMs showed ... ct town mapsWebContribute to zhengxj1/Seurat development by creating an account on GitHub. ct town job openingsWebNov 18, 2024 · The infiltration of macrophages, TLR9, autophagy proteins (TFEB and LC3) and inflammatory cytokines increased in the periodontitis-with-RA group and was reduced by the inhibition of Ctsk in the periodontal region. Macrophage stimulation confirmed the in … ease tax solutionsWebOct 1, 2001 · Cathepsin K (Ctsk) produced by osteoclasts, Cathepsin B (Ctsb) produced by septoclasts, macrophages, and osteoclasts, and immunostaining for the pan … ease taxWebApr 14, 2024 · Significant upregulation of several known OC genes was established in the OC group, such as CTSK, DC-STAMP, ACP5, MMP9, ATPV0D2, and ITGB3, which gives confidence in the OC-like nature of the cell culture model. In addition, several genes identified as upregulated in the OCs have genetic evidence for association with bone traits. easesystemsWebMay 19, 2024 · In humans, phagosomal CtsK activity was reduced in COPD lung macrophages and non-COPD lung macrophages exposed to cigarette smoke extract. … ease tablesWebFeb 2, 2024 · Odanacatib and Nitazoxanide, targeting CTSK and SIX1 respectively, both inhibit the alternative activation of undifferentiated macrophages, and MIC triggers the polarization of M1 macrophages. Meanwhile, nanoscale drug Ru @ ICG-BLZ NP can directly induce the conversion of M2 phenotype to M1 phenotype, showing a new method … ct town lines